Coding

Part:BBa_K2722000:Design

Designed by: Enikö Baligács   Group: iGEM18_Munich   (2018-10-03)


Exonuclease V, Subunit RecC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 118
    Illegal AgeI site found at 1971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein should preferable be tagged on the C-terminus. (5)

Source

This part was extrected from the Genome of E.coli (strain: Rosetta) via PCR and cloned into pSB1C3.

References

UniProt: 1w36 - RecBCD/DNA-complex

(1) Singleton, M. R., Dillingham, M. S., Gaudier, M., Kowalczykowski, S. C., & Wigley, D. B. (2004). Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature, 432(2014), 187.

(2) Smith, G. R. (2012). How RecBCD enzyme and Chi promote DNA break repair and recombination: a molecular biologist's view. Microbiology and Molecular Biology Reviews, 76(2), 217-228.

(3) Smith, G. R. (2001). Homologous recombination near and far from DNA breaks: alternative roles and contrasting views. Annual review of genetics, 35(1), 243-274.

(4) Dillingham, M. S., & Kowalczykowski, S. C. (2008). RecBCD enzyme and the repair of double-stranded DNA breaks. Microbiology and Molecular Biology Reviews, 72(4), 642-671.


(5) Taylor, A. F., Amundsen, S. K., Guttman, M., Lee, K. K., Luo, J., Ranish, J., & Smith, G. R. (2014). Control of RecBCD enzyme activity by DNA binding-and Chi hotspot-dependent conformational changes. Journal of molecular biology, 426(21), 3479-3499.